You can save your searches as RSS (Really Simple Syndication) feeds by running a search in PubMed, and clicking on the RSS link located below the search box:
Click on the Create RSS button to create the feed, and then on the XML button to see the results:
Copy and paste the URL into the subscribe form in your RSS reader, or choose an application from the drop-down menu and click Subscribe Now:
To save a search and set up an e-mail search alert, on the results page click Create alert:
A login page for My NCBI will open. Log in to My NCBI (or create an account), select e-mail delivery, choose the frequency and day for the alert, the format and number of items, add an optional message, and then click Save:
You will receive e-mail updates whenever articles that match your search are added to PubMed. Additionally the search will appear under Saved Searches when you log in to your My NCBI account.